High-throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae.
Liu X, Gallay C, Kjos M, Domenech A, Slager J, van Kessel SP, Knoops K, Sorg RA, Zhang JR, Veening JW
Mol Syst Biol. 2017 May 10;13(5):931. doi: 10.15252/msb.20167449.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85588 | pJWV102-PL-dCas9 | Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp |
| 85589 | pPEPY-PF6-lacI | Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae |
| 85590 | pPEPX-P3-sgRNAluc | Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for |