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Cortical F-actin stabilization generates apical-lateral patterns of junctional contractility that integrate cells into epithelia.

Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, Hamilton NA, Neufeld Z, Yap AS
Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 2014 Jan 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
101278pLL5.0 E-cadherin shRNA/Ecad-tdTomatoLentiviral expression of shRNA targeting Ecad and Ecad-tdtomato
101279pCS2.0 Tag-RFP-T-UtrCHLife imaging pCS2.0 Tag-RFP-T_UtrCH
101282pLL5.0 E-cadherin shRNA/mE-cadherin-mCherryLentiviral expression of shRNA and mouse E-cadherin -mCherry
101283pE-cadherin-GFP-N-WASP deltaVCAMammalian expression of E-cadherin-GFP-N-WASP delta VCA
101285pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP
101286pLL5.0 mCherry_K-RasC14Lentiviral expression of K-Ras C14

Antibodies from Article