Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona.
Gandhi S, Haeussler M, Razy-Krajka F, Christiaen L, Stolfi A
Dev Biol. 2017 May 1;425(1):8-20. doi: 10.1016/j.ydbio.2017.03.003. Epub 2017 Mar 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87542 | U6>XX | Component 1 of 2 for U6>sgRNA cassette OSO-PCR |
| 87543 | XX>sgRNA F+E | Template 2 (of 2) for U6>sgRNA cassette OSO-PCR |