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Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T
Cell. 2017 Jan 12;168(1-2):264-279.e15. doi: 10.1016/j.cell.2016.12.032. Epub 2017 Jan 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
968065HT6-YFP-ThymosinBeta4(WT)cilia-membrane-targeted G-actin-binding protein
968075HT6-YFP-ThymosinBeta4(KK18,19EE)non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)
968085HT6-YFP-Inpp5ecilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-null
968095HT6-YFP-Inpp5e(D477N)phosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-null
968105HT6-YFP-PIPKcilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase
968115HT6-YFP-PIPK(D253A)kinase-dead control for 5HT6-YFP-PIPK
968125HT6-YFP-Inp54pcilia-membrane-targeted inositol polyphosphate 5-phosphatase
968135HT6-YFP-Inp54p(D281A)phosphatase-dead control for 5HT6-YFP-Inp54p

Antibodies from Article