The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.
Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
RNA. 2009 May . 15(5):804-13.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 21514 | GST-TNR(aa1-204) | |
| 21516 | GST-GW1D10(aa566-1343) | |
| 21517 | GFP-GW1D10(aa566-1343) | |
| 21519 | GFP-GW1D5(aa1670-1962) | |
| 21520 | GFP-GW1D1a(aa254-503) | |
| 21521 | GFP-GW1D1b(aa502-751) | |
| 21522 | GFP-GW1D1aMut(W>A) | |
| 21523 | GST-GW1D1(aa254-751) | |
| 21524 | GFP-D1a_W1-2(aa270-346) | |
| 21525 | GFP-D1a_W2-3(aa318-399) | |
| 21526 | GFP-D1a_W3-4(aa340-439) | |
| 21527 | GFP-D1a_W4-5(aa409-495) | |
| 21528 | GST-GW1D12(aa895-1211) | |
| 21529 | FLAG-PAZ(aa1-480) | |
| 21530 | NHA-PAZ(aa1-480) | |
| 21531 | GFP-Ago3m | |
| 21532 | NHA-Ago3m | |
| 21533 | FLAG-Ago1 | |
| 21534 | GFP-Ago1 | |
| 21535 | NHA-Ago1 | |
| 21536 | GFP-Ago4 | |
| 21537 | NHA-Ago4 | |
| 21538 | FLAG-Ago2 | |
| 21541 | NHA-PIWI(aa478-860) | |
| 21542 | GST-PIWI(aa478-860) | |
| 21543 | FLAG-PIWI(aa478-860) |