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Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency.

Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
Nat Methods. 2009 May . 6(5):370-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
21312PL-SIN-PGK-EiPControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance
21313PL-SIN-EOS-C(3+)-EiPLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
21314PL-SIN-EOS-S(4+)-EiPLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance
21315pMXs-mRFP1MoMLV-based Retroviral vector, expresses mRFP1
21316PL-SIN-PGK-EGFPControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP
21317PL-SIN-EOS-S(4+)-EGFPLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP
21318PL-SIN-EOS-C(3+)-EGFPLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP
21319PL-SIN-Oct4-EGFPLentiviral vector, expresses EGFP under Oct3/4 promoter
21320PL-SIN-EF1α-EGFPControl Lentiviral vector with strong EF1a promoter, expresses EGFP
21321PL-SIN-Nanog-EGFPLentiviral vector, expresses EGFP under Nanog promoter

Antibodies from Article