Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency.

Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
Nat Methods. 2009 May . 6(5):370-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
21321	PL-SIN-Nanog-EGFP	Lentiviral vector, expresses EGFP under Nanog promoter
21320	PL-SIN-EF1α-EGFP	Control Lentiviral vector with strong EF1a promoter, expresses EGFP
21319	PL-SIN-Oct4-EGFP	Lentiviral vector, expresses EGFP under Oct3/4 promoter
21318	PL-SIN-EOS-C(3+)-EGFP	Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP
21317	PL-SIN-EOS-S(4+)-EGFP	Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP
21316	PL-SIN-PGK-EGFP	Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP
21315	pMXs-mRFP1	MoMLV-based Retroviral vector, expresses mRFP1
21314	PL-SIN-EOS-S(4+)-EiP	Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance
21313	PL-SIN-EOS-C(3+)-EiP	Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
21312	PL-SIN-PGK-EiP	Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance