CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S
Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85542 | pJYS3_ΔcrtYf | Constitutive transcription of FnCpf1 and crRNA of crtYf |
| 85543 | pJYS2cm_crtYf | Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance |
| 85544 | pJYS2_crtYf | Constitutive transcription of crRNA of crtYf, Spectinomycin resistance |
| 85545 | pJYS1Ptac | Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter |
| 85546 | pJYS1Peftu | Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter |
| 89077 | pJYS2.1-crtYf | Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum. |