Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions.

Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi M, Drowley L, Plowright A, Wang QD, Goumans MJ, Palecek SP
Nat Biomed Eng. 2016;1. pii: 0003. doi: 10.1038/s41551-016-0003. Epub 2016 Dec 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
82333	WT1-2A-eGFP PGK PuroR Donor Plasmid	WT1 targeting donor
92312	WT1 KI gRNA1	CRISPR-GFP-gRNA for cutting WT1
92313	WT1 KI gRNA2	CRISPR-GFP-gRNA for cutting WT1