An Optical Neuron-Astrocyte Proximity Assay at Synaptic Distance Scales.
Octeau JC, Chai H, Jiang R, Bonanno SL, Martin KC, Khakh BS
Neuron. 2018 Apr 4;98(1):49-66.e9. doi: 10.1016/j.neuron.2018.03.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92277 | pcDNA3.1 CMV NAPA-A BGH | FRET probe; extracellular membrane tethered eGFP |
| 92278 | pcDNA 3.1 CMV NAPA-N BGH | FRET probe; extracellular synapse targeted mCherry |
| 92280 | pcDNA3.1 CMV mCherry-eGFP BGH | Control FRET construct; tandem mCherry-eGFP fusion |
| 92281 | pZac2.1 GfaABC1D NAPA-A SV40 | FRET probe; extracellular membrane tethered eGFP |
| 92282 | pZac2.1 hSynapsin1 NAPA-N SV40 | FRET probe; extracellular synapse targeted mCherry |
| 92283 | pZac2.1 hSynapsin1 FLEX NAPA-N SV40 | FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed |
| 97212 | pZac2.1 hSynapsin1 NAPA-N2 SV40 | FRET probe; extracelllular synapse targeted mScarlet |