Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle.
Papagiannakis A, Niebel B, Wit EC, Heinemann M
Mol Cell. 2017 Jan 19;65(2):285-295. doi: 10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92179 | pTEF:ATP | HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration. |
| 102882 | pG2J | Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation. |
| 102883 | pOsTIR1w/oGFP | Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation. |
| 102884 | pG23A | Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast. |