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Structural and kinetic characterization of quinolinate phosphoribosyltransferase (hQPRTase) from homo sapiens.

Liu H, Woznica K, Catton G, Crawford A, Botting N, Naismith JH
J Mol Biol. 2007 Oct 26;373(3):755-63. Epub 2007 Aug 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92166pEHIShqprtPlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
92403pEHIShqprtK139AhQPRTase harbouring K139A mutation
92404pEHIShqprtK171ShQPRTase harbouring K171S mutation
92405pEHIShqprtR102AhQPRTASE harbouring R102A mutation
92407pEHIShqprtR102QhQPRTase harboring R102Q mutation
92411pEHIShqprtR138QhQPRTase harbouring R138Q mutation
92412pEHIAhqprtR161GhQPRTase harbouring R161G mutation
92413pEHIShqprtR161QhQPRTase harboring R161Q mutation

Antibodies from Article