Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism.
VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 10.1074/jbc.M117.785287.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73741 | pET151-HIS-TEV-GST-PP-RPN13 | Expression of hRPN13 in E.coli |
| 73742 | pET151-GST-PP-RPN13 (19-132) | Expression of human RPN13 PRU domain in E.coli |
| 73743 | pET151-GFP-PP-RPN2 (918-953) | Expression of GFP-PP-RPN2 (918-53) fusion in E.coli |
| 73744 | pET151-GFP-PP-RPN2 (925-953) | Expression of GFP-PP-RPN2 (925-953) fusion in E.coli |
| 73745 | pET151-GFP-PP-RPN2 (932-953) | Expression of GFP-PP-RPN2 (932-953) fusion in E.coli |
| 73746 | pET151-GFP-PP-RPN2 (940-953) | Expression of GFP-PP-RPN2 (940-953) fusion in E.coli |
| 73747 | pET151-GFP-PP-RPN2 (918-947) | Expression of GFP-PP-RPN2 (918-947) fusion in E.coli |
| 73748 | pET151-GFP-PP-RPN2 (918-932) | Expression of GFP-PP-RPN2 (918-932) fusion in E.coli |
| 74228 | pET151-GFP-PP-RPN2 (797-953) | [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli |