Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.
Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY
Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92149 | pLX_TRC208-SYNJ2BP-V5 | Expresses V5-tagged human SYNJ2BP in mammalian cells. |
| 92150 | pcDNA4 HisMax-V5-GFP-RRBP1 | Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells. |
| 92156 | lentiGuide-RRBP1-1 | CRISPR guide RNA targeting human RRBP1 |
| 92157 | lentiGuide-RRBP1-2 | CRISPR guide RNA targeting human RRBP1 |
| 92158 | pLX304-Flag-APEX2-NES | Cytosolic APEX2 construct |
| 92159 | lentiGuide-SYNJ2BP-1 | CRISPR guide RNA targeting human SYNJ2BP |
| 92160 | lentiGuide-SYNJ2BP-2 | CRISPR guide RNA targeting human SYNJ2BP |