Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids.
Schlesinger O, Chemla Y, Heltberg M, Ozer E, Marshall R, Noireaux V, Jensen MH, Alfonta L
ACS Synth Biol. 2017 Mar 9. doi: 10.1021/acssynbio.7b00019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92222 | pBEST-p70b-UTR1-deGFP-6xHis-T500 | Expression of deGFP using the p70b promoter (weak version of the p70a promoter) |
| 92224 | pBEST-p70a-UTR3-deGFP-6xHis-T500 | Expression of deGFP using the p70a promoter and UTR3 |
| 92225 | pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500 | Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1 |
| 92227 | pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500 | Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1 |
| 92228 | pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500 | Expression of zmADH using the p70a promoter and UTR1 |
| 92229 | pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500 | Expression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1 |
| 92230 | pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500 | Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1 |
| 92314 | pBEST-p70a-UTR1-Nterm 6xHis PL-T500 | Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1 |
| 92315 | pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500 | Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1 |
| 92316 | pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500 | Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1 |
| 92317 | pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500 | Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3 |
| 92318 | pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500 | Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1 |
| 92319 | pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500 | Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3 |