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BLaTM 2.0, a genetic tool revealing preferred antiparallel interaction of transmembrane helix 4 of the dual-topology protein EmrE.

Julius A, Laur L, Schanzenbach C, Langosch D
J Mol Biol. 2017 Apr 18. pii: S0022-2836(17)30166-3. doi: 10.1016/j.jmb.2017.04.003. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92060C-BLa 2.0The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.
207846NBla_1.2_EmrE_TMD4_3PBLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vector
207847NBla_1.2_EmrE_TMD4_3P_G90V_G97VBLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vector
207848CBla_2.0_EmrE_TMD4_3PBLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector
207849CBla_2.0_EmrE_TMD4_3P_G90V_G97VBLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in CBLa 2.0 vector

Antibodies from Article