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A transcription activator-like effector (TALE) induction system mediated by proteolysis.

Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF
Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92016p5A0-TExpresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease
92017p5A1-TExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
92018p5A1-TiExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease
92019p5A3-TExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
92020p5A7-TExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
92021p5B3-TExpresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
92022p5C3-TExpresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
92023p5mCherrymCherry reporter plasmid with lac operator in its promoter
92024p5sfGFPsfGFP reporter plasmid with lac operator in its promoter
92025p5lysAsfGFPsfGFP reporter plasmid with lysA TALE binding site in its promoter
92026p5sucAmCherrymCherry reporter plasmid with sucA TALE binding site in its promoter

Antibodies from Article