An Argonaute phosphorylation cycle promotes microRNA-mediated silencing.
Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT
Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 91975 | MSCV-EGFP | EGFP reporter cell line control (no miR-19 sites) |
| 91976 | MSCV-EGFP-Bulged-miR-19 | EGFP reporter cell line with eight bulged miR-19 sites |
| 91977 | LcV2-Hygro | LentiCRISPR v2 with Hygro cloned in place of Puro |
| 91978 | pLJM1-FH-AGO2-WT | Expresses FLAG-HA-AGO2 (WT) |
| 91979 | pLJM1-FH-AGO2-5xA | Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)] |
| 91980 | pLJM1-Empty | Empty backbone |
| 91981 | pLJM1-FH-AGO2-5xE | Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)] |
| 91982 | pLJM1-FH-AGO2-S828A | Expresses FLAG-HA-AGO2 (S828A) |
| 91983 | pLJM1-FH-AGO2-D358K | Expresses FLAG-HA-AGO2 (D358K) |
| 91984 | pLJM1-FH-AGO2-R438E | Expresses FLAG-HA-AGO2 (R438E) |
| 91985 | pLJM1-FH-AGO2-K525E | Expresses FLAG-HA-AGO2 (K525E) |
| 91986 | MSCV-Puro-FH-AGO2-WT | Expresses FLAG-HA-AGO2 (WT) |
| 91987 | MSCV-Puro-FH-AGO2-S824A | Expresses FLAG-HA-AGO2 (S824A) |
| 91988 | MSCV-Puro-FH-AGO2-T830A | Expresses FLAG-HA-AGO2 (T830A) |
| 91989 | MSCV-Puro-FH-AGO2-S831A | Expresses FLAG-HA-AGO2 (S831A) |
| 91990 | MSCV-Puro-FH-AGO2-S834A | Expresses FLAG-HA-AGO2 (S834A) |
| 91991 | MSCV-Puro-FH-AGO2-S828A | Expresses FLAG-HA-AGO2 (S828A) |
| 91992 | MSCV-Puro-FH-AGO2-5xA | Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)] |
| 91993 | MSCV-Puro-FH-AGO2-5xE | Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)] |
| 91994 | MSCV-Puro-FH-AGO2-S824E | Expresses FLAG-HA-AGO2 (S824E) |
| 91995 | MSCV-Puro-FH-AGO2-S828E | Expresses FLAG-HA-AGO2 (S828E) |
| 91996 | MSCV-Puro-FH-AGO2-T830E | Expresses FLAG-HA-AGO2 (T830E) |
| 91997 | MSCV-Puro-FH-AGO2-S831E | Expresses FLAG-HA-AGO2 (S831E) |
| 91998 | MSCV-Puro-FH-AGO2-S834E | Expresses FLAG-HA-AGO2 (S834E) |
| 91999 | MSCV-Puro-FH-AGO2-S824E-S828E | Expresses FLAG-HA-AGO2 (S824E-S828E) |
| 92000 | MSCV-Puro-FH-AGO2-S824E-S831E | Expresses FLAG-HA-AGO2 (S824E-S831E) |
| 92001 | MSCV-Puro-FH-AGO2-S828E-S831E | Expresses FLAG-HA-AGO2 (S828E-S831E) |
| 92002 | MSCV-Puro-FH-AGO2-S824E-S828E-S831E | Expresses FLAG-HA-AGO2 (S824E-S828E-S831E) |
| 92003 | MSCV-Puro-FH-AGO2-S824A-T830A | Expresses FLAG-HA-AGO2 (S824A-T830A) |
| 92004 | pGL3-BoxB | Expresses firefly luciferase with BoxB sequences in 3' UTR |
| 92005 | pcDNA3.1+_Lambda-N-HA-Peptide | Expresses Lambda-N-HA peptide |
| 92006 | pcDNA3.1+_FH-AGO2-WT | Expresses FLAG-HA-AGO2 (WT) |
| 92007 | pcDNA3.1+_FH-AGO2-5xA | Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)] |
| 92008 | pcDNA3.1+_FH-AGO2-5xE | Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)] |
| 92009 | pcDNA3.1+_LN-HA-AGO2-WT | Expresses Lambda-N-HA-AGO2 (WT) |
| 92010 | pcDNA3.1+_LN-HA-AGO2-5xA | Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)] |
| 92011 | pcDNA3.1+_LN-HA-AGO2-5xE | Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)] |
| 92012 | pcDNA3.1+_V5-ANKRD52 | Expresses V5-ANKRD52 |
| 92013 | pCAGIG_V5-PPP6C | Expresses V5-PPP6C, includes IRES-Emerald |
| 92014 | pCAGImC_V5-CSNK1A1 | Expresses V5-CSNK1A1, includes IRES-mCherry |
| 92015 | pCAGImC_Empty | Empty backbone, includes IRES-mCherry |