Marker-free coselection for CRISPR-driven genome editing in human cells.

Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y
Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
86551	ATP1A1_plasmid_donor_RD	Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
86610	eSpCas9(1.1)_No_FLAG_ATP1A1_G2	Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid
86611	eSpCas9(1.1)_No_FLAG_ATP1A1_G3	Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
86612	eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA	Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
86613	eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA	Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
86617	pY036_ATP1A1_G3	Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1
86618	pY036_ATP1A1_G5	Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1
86619	pY036_ATP1A1_G3_Array	Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
86620	pY036_ATP1A1_G5_Array	Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid