A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription.

Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, Whitby FG, Formosa T, Hill CP
Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
91820	CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)	Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
91819	CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)	Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
91818	CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)	Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations
91817	CPH3495 (pET151_Spt6 1247-1451 K1435A)	Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation
91816	CPH3567 (pET151_Spt6 1247-1451 R1282H)	Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation
91815	CPH1141 (pET151_Spt6 1247-1451)	Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6
91814	CPH3566 (pDEST15_Spt6 1223-1451)	Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6
91813	CPH3511 (YCp LEU2 Rpb1 P1472A)	Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation
91812	CPH3509 (YCp LEU2 Rpb1 T1471A)	Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation
91811	CPH3508 (YCp LEU2 Rpb1 Y1473A)	Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation