A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription.
Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, Whitby FG, Formosa T, Hill CP
Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 91806 | CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP) | Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site |
| 91807 | CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP) | Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site |
| 91808 | CPH3486 (YCp LEU2 Rpb1 F1492A) | Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation |
| 91809 | CPH3487 (YCp LEU2 Rpb1 S1493A) | Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation |
| 91810 | CPH3488 (YCp LEU2 Rpb1 P1494A) | Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation |
| 91811 | CPH3508 (YCp LEU2 Rpb1 Y1473A) | Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation |
| 91812 | CPH3509 (YCp LEU2 Rpb1 T1471A) | Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation |
| 91813 | CPH3511 (YCp LEU2 Rpb1 P1472A) | Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation |
| 91814 | CPH3566 (pDEST15_Spt6 1223-1451) | Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6 |
| 91815 | CPH1141 (pET151_Spt6 1247-1451) | Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 |
| 91816 | CPH3567 (pET151_Spt6 1247-1451 R1282H) | Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation |
| 91817 | CPH3495 (pET151_Spt6 1247-1451 K1435A) | Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation |
| 91818 | CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A) | Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations |
| 91819 | CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1) | Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD |
| 91820 | CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2) | Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD |