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CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis.

Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L
Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
91707pHEE901CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR
91708pHSE901CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR
91709pBSE901CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance
91713pXSE901BCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance
91714pXSE901BGCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

Antibodies from Article