A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.
Zhang M, Lin S, Song X, Liu J, Fu Y, Ge X, Fu X, Chang Z, Chen PR
Nat Chem Biol. 2011 Sep 4;7(10):671-7. doi: 10.1038/nchembio.644.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
91705 | pSupAR-MbPylRS(DiZPK) | express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest |