Structural Basis of Protein Translocation by the Vps4-Vta1 AAA ATPase.
Monroe N, Han H, Shen PS, Sundquist WI, Hill CP
Elife. 2017 Apr 5;6. pii: e24487. doi: 10.7554/eLife.24487.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87733 | pET151-Vps4 | Bacterial expression of full-length S. cerevisiae Vps4 |
| 87734 | pET151-Vps4(81-437) | Bacterial expression of N-terminally truncated S. cerevisiae Vps4 |
| 87735 | pET151-Vps4(101-437) | Bacterial expression of N-terminally truncated S. cerevisiae Vps4 |
| 87736 | pET151-Vps4(101-437) L151D | Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette |
| 87737 | pET151-Vps4(101-437)-Hcp1 | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |
| 87738 | pET151-Vta1(280-330) | Bacterial expression of the VSL domain of S. cerevisiae Vta1 |
| 87739 | pET151-Vta1(280-330) S278C | Bacterial expression of a VSL domain variant of S. cerevisiae Vta1 |
| 87747 | pET151-Vps4(101-437)-Hcp1 K321A | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |
| 87748 | pET151-Vps4(101-437)-Hcp1 E322A | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |
| 87749 | pET151-Vps4(101-437)-Hcp1 R325A | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |
| 89287 | pET151-Vps4(101-437)-Hcp1 K321A/R325A | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |
| 89288 | pET151-Vps4(101-437)-Hcp1 K321D | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |
| 89289 | pET151-Vps4(101-437)-Hcp1 R325D | Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1 |