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Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation.

Daeffler KN, Galley JD, Sheth RU, Ortiz-Velez LC, Bibb CO, Shroyer NF, Britton RA, Tabor JJ
Mol Syst Biol. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
90952pKD182Expresses ThsS under the Ptac promoter
90953pKD184Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively
90954pKD227Expresses S. baltica TtrS under the Ptac promoter
90955pKD233.7-3Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively
90956pKD236-4bExpresses ThsS constitutively
90957pKD237-3a-2Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter
90958pKD238-1aExpresses S. baltica TtrS constitutively
90959pKD239-1g-2Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

Antibodies from Article