A FRET sensor enables quantitative measurements of membrane charges in live cells.
Ma Y, Yamamoto Y, Nicovich PR, Goyette J, Rossy J, Gooding JJ, Gaus K
Nat Biotechnol. 2017 Mar 13. doi: 10.1038/nbt.3828.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90411 | MCS Lck only | A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair. |
| 90412 | MCS+ | A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane |
| 90413 | MCS Hras FI | A control of MCS+ that associated with cell plasma membrane by two hydrophobic interaction at N and C terminus of the FRET pair. |