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Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast.

Abelson J, Hadjivassiliou H, Guthrie C
Methods Enzymol. 2010;472:31-40. doi: 10.1016/S0076-6879(10)72017-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111322pre-Ubc4 BrG pUC19for transcription to Ubc4 pre-mRNA

Antibodies from Article