Live-cell measurements of kinase activity in single cells using translocation reporters.
Kudo T, Jeknic S, Macklin DN, Akhter S, Hughey JJ, Regot S, Covert MW
Nat Protoc. 2018 Jan;13(1):155-169. doi: 10.1038/nprot.2017.128. Epub 2017 Dec 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90227 | pLentiPGK Puro DEST ERKKTRClover | Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance) |
| 90229 | pLentiPGK Hygro DEST ERKKTRmCerulean3 | Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance) |
| 90231 | pLentiPGK Blast DEST ERKKTRmRuby2 | Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance) |
| 90232 | pLentiPGK Puro DEST JNKKTRmCerulean3 | Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance) |
| 90234 | pLentiPGK Hygro DEST H2B-mCerulean3 | Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance) |
| 90236 | pLentiPGK Hygro DEST H2B-mRuby2 | Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance) |
| 90237 | pLentiPGK DEST H2B-iRFP670 | Lentiviral vector to express H2B-iRFP670 under PGK promoter |
| 90238 | pLentiPGK Puro DEST JNKKTR AA Clover | Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance) |
| 90239 | pLentiPGK Puro DEST JNKKTR EE Clover | Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance) |
| 90240 | pLentiPGK Puro DEST JNKKTR AE Clover | Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance) |