Real-time detection of N-end rule-mediated ubiquitination via fluorescently labeled substrate probes.

Mot AC, Prell E, Klecker M, Naumann C, Faden F, Westermann B, Dissmeyer N
New Phytol. 2017 Mar 9. doi: 10.1111/nph.14497. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
83391	His8:MBP-tev-Tyr-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83392	His8:MBP-tev-Ile-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83393	His8:MBP-tev-Ala-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies