Multimodal fast optical interrogation of neural circuitry.
Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K
Nature. 2007 Apr 5. 446(7136):633-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 20938 | pcDNA3.1/hChR2(H134R)-mCherry | Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation |
| 20939 | pcDNA3.1/hChR2-EYFP | Mammalian expression of humanized ChR2 fused to EYFP |
| 20940 | pcDNA3.1/hChR2(H134R)-EYFP | Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation |
| 20942 | pLenti-EF1a-hChR2(H134R)-EYFP-WPRE | 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation |
| 20943 | pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE | 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation |
| 20944 | pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE | 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation |
| 20945 | pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE | 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation |
| 20946 | pLenti-CaMKIIa-eNpHR-EYFP-WPRE | 3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition |