Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.
Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A, Kunieda T
Nat Commun. 2016 Sep 20;7:12808. doi: 10.1038/ncomms12808.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90019 | pCXN2KS-Dsup | To establish stable line expressing Dsup in constitutive manner |
| 90020 | pAcGFP1-N1-Dsup | To express GFP-tagged Dsup in mammalian cells transiently |
| 90021 | pCold-I-Dsup | To express His-tagged Dsup in bacteria |
| 90022 | pAcGFP1-N1-Dsup-C | To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently |
| 90023 | pAcGFP1-N1-Dsup-deltaC | To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently |
| 90024 | pLKO.1-shDsup | To establish Dsup knock down cells |