Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.
Goldfarb KC, Cech TR
Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub 2017 Jan 23.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
87189 | px330-MRPsg1 | disruption of human RMRP gene via CRISPR-Cas9, guide 1 |
87190 | px330-MRPsg2 | disruption of human RMRP gene via CRISPR-Cas9, guide 2 |
87191 | px330-MRPsg3 | disruption of human RMRP gene via CRISPR-Cas9, guide 3 |
87192 | px330-MRPsg4 | disruption of human RMRP gene via CRISPR-Cas9, guide 4 |
87193 | psglessClonePX | used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice |
87194 | p_3xFLAG-Rpp25 | Expression of ectopic 3xFLAG-Rpp25 construct from CMV promoter |
87199 | p-715MRPprom-MRPcucuc | Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency |