Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28.
Smargon AA, Cox DB, Pyzocha NK, Zheng K, Slaymaker IM, Gootenberg JS, Abudayyeh OA, Essletzbichler P, Shmakov S, Makarova KS, Koonin EV, Zhang F
Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89898 | pBZCas13b | Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI. |
| 89900 | pBZcsx27 | Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter |
| 89901 | pBZCas13b-HEPN | Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI. |
| 89906 | pPbcas13b | Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI. |
| 89909 | pPbcsx28 | Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter |