Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to oncogene activation in melanocytes.
Kenyon A, Gavriouchkina D, Zorman J, Chong-Morrison V, Napolitani G, Cerundolo V, Sauka-Spengler T
Dis Model Mech. 2018 Apr 6;11(4). pii: dmm.030056. doi: 10.1242/dmm.030056.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89886 | pCrysβ:ECFP-LexOP:mCherry-NRasQ61K | Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening. |
| 89887 | pCrysβ:ECFP-LexOP:mCherry-HrasG12V | Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening. |
| 89888 | pCrysβ:ECFP-LexOP:mCherry-KrasG12V | Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening. |
| 89890 | pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT | BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette. |
| 110201 | pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences, | Melanocyte-specific expression of the LexPR-2A-Cerulean fusion |