Optogenetic control with a photocleavable protein, PhoCl.

Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
87699	pcDNA-mCherry-4A/4B-CAAX	Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells
87698	pcDNA-PANX1(HCVp)	Express HCV protease-activatible pannexin-1 in mammalian cells
87697	pcDNA-I-PhoCl-HCVp	Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells
87696	pUAS-NanoLuc	Gal4VP16 driven expression of NanoLuc
87695	pUAS-mCherry-NLS	Gal4VP16 driven expression of mCherry-NLS in mammalian cells
87694	pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2	Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells
87693	pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2	Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells
87692	pcDNA-PhoCl-mCherry-myc	Express PhoCl-mCherry with C-term myc tag in mammalian cells
87691	pcDNA-NLS-PhoCl-mCherry	Express NLS-PhoCl-mCherry in mammalian cells
87690	pcDNA-NES-PhoCl-mCherry	Express NES-PhoCl-mCherry in mammalian cells