Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis.

Gilbert C, Howarth M, Harwood CR, Ellis T
ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
87370	pCG-A-sacB(SP)-B	An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
87371	pCG-A-celA(SP)-B	An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
87372	pCG-A-xynA(SP)-B	An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
87373	pCG-B-SpyTag-C	An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly
87374	pCG-B-mSpyCatcher-C	An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly
87375	pCG-D-SpyTag-His6-E	An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly
87376	pCG-D-mSpyCatcher-His6-E	An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass
87377	pCG004	ORF part assembly entry vector derived from pHT01.