A genetic system to study Plasmodium falciparum protein function.
Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85789 | pSLI-2xFKBP-GFP(1463000) | P. falciparum integration plasmid to fuse endogenous genes with tags using SLI |
| 85790 | pSLI-sandwich(0807600) | P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI |
| 85791 | pSLI-TGD(1463000) | P. falciparum integration plasmid to disrupt genes using SLI |
| 85792 | pSLI-N-GFP-2xFKBP-loxP(K13) | P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI |
| 85793 | pSLI-N-sandwich-loxP(K13) | P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI |
| 85795 | p1xNLS-FRB-mCherry-hsp86-BSD | Expression of 1xNLS mislocaliser in P. falciparum |
| 85796 | pLyn-FRB-mCherry-nmd3-BSD | Expression of PPM mislocaliser in P. falciparum |
| 85797 | pSkipFlox | P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide |