Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.
Lin L, Petersen TS, Jensen KT, Bolund L, Kuhn R, Luo Y
J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87263 | P628_SpCas9-RecA | Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency |
| 87264 | P823_eSpCas9(1.1)-RecA | Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency |