MEF2C Phosphorylation Is Required for Chemotherapy Resistance in Acute Myeloid Leukemia.

Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA, Armstrong SA, Kentsis A
Cancer Discov. 2018 Apr;8(4):478-497. doi: 10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
89715	MSCV-IRES-Tomato MEF2C	Retroviral gene expression vector for MEF2C expression
89716	MSCV-IRES-Tomato MEF2C S222A	Retroviral gene expression vector for MEF2C-S222A expression
89717	pINDUCER20 MEF2C	Lentiviral gene expression vector for doxycycline inducible MEF2C expression
89718	pINDUCER20 MEF2C S222A	Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression
107229	MSCV-IRES_Tomato	Tomato expressing plasmid
107231	MSCV-IRES_Tomato MEF2C S222D	hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis
107232	pLT3REVIR_MARK3 1122	shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one
107233	pLT3REVIR_MARK3 2573	shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one
107234	pINDUCER20 MEF2C S222D	MEF2C phosphomimetic mutant serine to aspartic acid
107235	pLX304-MARK3	Lentiviral gene expression vector for phospho-MARK3 expression
107236	pLX304-MARK3_T211A	Lentiviral gene expression vector for phospho-MARK3_T221A expression
107238	pRecLV105	A lentiviral vector for expression in mammalian cells
107239	pRecLV105 MEF2C S222A	A lentiviral vector for expression in mammalian cells encoding MEF2C-S222A insert
107240	pRecLV105 MEF2C	A lentiviral vector for expression in mammalian cells encoding MEF2C insert