Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.
Zamora CY, Schocker NS, Chang MM, Imperiali B
Methods Enzymol. 2017;597:145-186. doi: 10.1016/bs.mie.2017.06.003. Epub 2017 Jul 5.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
89708 | GST-PglF130 | Expresses truncated PglF from C. jejuni with N-terminal GST tag |
89711 | T7-WbpB-His6 | Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag |
89712 | T7-WbpE-His6 | Expresses WbpE from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag |
89713 | T7-WbpD-His6 | Expresses WbpD from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag |
89714 | T7-AglK-His | Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression. |
89723 | T7-PseB-His6 | Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag |
89724 | His8-TEV-PseC | Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site |
89725 | His8-TEV-PseH | Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site |
89726 | T7-AglC-His6 | Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression. |