Tunable thermal bioswitches for in vivo control of microbial therapeutics.
Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG
Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. Epub 2016 Nov 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85455 | pTlpA-Wasabi | mWasabi reporter gene gated by wild type TlpA |
| 86100 | pTlpA-Wasabi-NF | Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA |
| 86101 | pTcI-Wasabi | mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI |
| 86102 | pLacI241-Wasabi | mWasabi reporter gene gated by temperature-sensitive LacI A241T |
| 86103 | pLacI265-Wasabi | mWasabi reporter gene gated by temperature-sensitive LacI G265D |
| 86104 | pTetR89-Wasabi | mWasabi reporter gene gated by temperature-sensitive TetR A89D |
| 86105 | pTetR193-Wasabi | mWasabi reporter gene gated by temperature-sensitive TetR I193N |
| 86107 | pRpoH-Wasabi | mWasabi reporter gene driven by RpoH promoter |
| 86108 | pClp-Wasabi | mWasabi reporter gene driven by ClpP/ClpX promoter |
| 86109 | pHtpG-Wasabi | mWasabi reporter gene driven by HtpG promoter |
| 86110 | pDnaK-Wasabi | mWasabi reporter gene driven by DnaK promoter |
| 86111 | pGrpE-Wasabi | mWasabi reporter gene driven by GrpE promoter |
| 86112 | pLacIq-Wasabi | mWasabi reporter gene driven by LacIq promoter |
| 86113 | pTlpAsp-Wasabi | mWasabi reporter gene driven by TlpA promoter with scrambled Pribnow box |
| 86114 | pTlpAreverse-Wasabi | mWasabi reporter gene driven by TlpA promoter in reverse orientation |
| 86115 | pTlpA36-Wasabi | mWasabi reporter gene gated by TlpA36 |
| 86116 | pTlpA39-Wasabi | mWasabi reporter gene gated by TlpA39 |
| 86117 | pTcI38-Wasabi | mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38 |
| 86118 | pTcI42-Wasabi | mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42 |
| 86119 | pCali2 | Multiplex temperature-gated expression of mWasabi and mCherry reporters |
| 86120 | pThermeleon | Bandpass temperature-gated expression of mWasabi and mCherry reporters |