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A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.

Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE, Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins JJ
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
89576pJK-caCas9-NatMX-Neut5LcaCas9 integrating vector into the Neut5L locus
89577pJK-caCas9-NatMX-Neut5L-Ade2 drivecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus
89578pJK-caCas9-NatMX-Neut5L-Leu2 drivecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Antibodies from Article