In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni.
Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim JS
Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89752 | pRGEN-CMV-CjCas9 | Expression of CjCas9 in mammalian cells |
| 89753 | pU6-Cj-sgRNA | U6 promoter driven expression of Cj-SgRNA cloned with BsmbI |
| 89754 | pET-CjCas9 | Expression of CjCas9 with His tag in E.coli |
| 137929 | pAAV-EFS-CjCas9-eGFP-HIF1a | Expression of cjCas9 with Hif1a targeting sgRNA |