Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K
Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80766 | pDonor-tBFP-NLS-Neo | Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system. |
| 80767 | pDonor-tBFP-NLS-Neo (Universal) | Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system. |
| 80768 | peSpCas9(1.1)-2×sgRNA (empty, donor) | All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty. |
| 80769 | peSpCas9(1.1)-2×sgRNA (IFT88, donor) | Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal). |