Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis.

Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M
Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
68369	EPB104	contains human U6 promoter
68370	U6GRNA	gRNA scaffold with hU6 promoter
68371	CAS9PBKS	CbAkt promoter driven Cas9-2A-GFP (from px458)