Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning.
Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 81163 | pSALECTNK-TEM1(S70A,D179G)/csTEM1 | pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1 |
| 81164 | pSALECTNK-LGK/csTEM1 | pSALECT plasmid with levoglucosan kinase fused to TEM-1 |
| 81165 | pETconNK-LGK | pETconNK with levoglucosan kinase |
| 81166 | pETconNK-TEM1(S70A,D179G) | pETconNK with TEM1(S70A,D179G) beta-lactamase |
| 81169 | pETconNK | pETconNK Empty Backbone |