In vitro reconstitution of the human RISC-loading complex.
MacRae IJ, Ma E, Zhou M, Robinson CV, Doudna JA
Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):512-7. doi: 10.1073/pnas.0710869105. Epub 2008 Jan 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89144 | pFastBac-hDicer | Expression of hDicer in Sf9 Cells |
| 89145 | pFastBac-hDcr-K70A. | Expression of helicase mutant of human Dicer in Sf9 cells |
| 89146 | pFastBac-Giardia Dicer | Expression of Giardia Dicer in Sf9 cells |