Facile manipulation of protein localization in fission yeast through GBP-GFP binding.
Chen YH, Wang GY, Hao HC, Chao CJ, Wang Y, Jin QW
J Cell Sci. 2017 Jan 12. pii: jcs.198457. doi: 10.1242/jcs.198457.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
89067 | pFA6a-GBP-kanMX6 | genomic GBP epitope C-terminal tagging in S. pombe |
89068 | pFA6a-GBP-mCherry-kanMX6 | genomic GBP-mCherry epitope C-terminal tagging in S. pombe |
89069 | pJK148-Pnmt41-GBP-mCherry(C)-leu1+ | genomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombe |
89070 | pJK148-Pnmt41-GBP-mCherry(N)-leu1+ | genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe |
89071 | pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1* | genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe |
89072 | pUC119-Padh81-GBP-mCherry(N)-hphMX6-lys1* | genomic GBP-mCherry epitope N-terminal tagging at lys1+ locus in S. pombe |