Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 88914 | pMJ915v2+Nterm Spe1 +Cterm Age1 site | For expression of modified SpyCas9 in e.coli. |
| 88915 | 1xNLS-pMJ915v2 | For expression of modified SpyCas9 in e.coli. |
| 88916 | 2xNLS-pMJ915v2 | For expression of modified SpyCas9 in e.coli. |
| 88917 | 4xNLS-pMJ915v2 | For expression of modified SpyCas9 in e.coli. |
| 88918 | pMJ915v2 + sfGFP | For expression of modified SpyCas9 in e.coli. |
| 88919 | 1xNLS-pMJ915v2-sfGFP | For expression of modified SpyCas9 in e.coli. |
| 88920 | 2xNLS-pMJ915v2-sfGFP | For expression of modified SpyCas9 in e.coli. |
| 88921 | 4xNLS-pMJ915v2-sfGFP | For expression of modified SpyCas9 in e.coli. |
| 88922 | 7xNLS-pMJ915v2-sfGFP | For expression of modified SpyCas9 in e.coli. |