Fluorescence Amplification Method for Forward Genetic Discovery of Factors in Human mRNA Degradation.
Alexandrov A, Shu MD, Steitz JA
Mol Cell. 2017 Jan 5;65(1):191-201. doi: 10.1016/j.molcel.2016.11.032. Epub 2016 Dec 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85442 | Launchpad (AVA2590) | Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette |
| 85443 | Fireworks RFP[PTC-] (AVA2515) | Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter. |
| 85444 | Fireworks RFP[PTC+] (AVA2626) | Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter. |
| 85445 | Fireworks GFP[PTC-] (AVA2598) | Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter. |
| 85446 | Fireworks GFP[PTC+] (AVA2600) | Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter. |