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Addgene

A comprehensive library of human transcription factors for cell fate engineering.

Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal M, Melero-Martin JM, Busskamp V, Church GM
Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 10.1038/s41587-020-0742-6. Epub 2020 Nov 30. (Link opens in a new window) PubMed (Link opens in a new window) Article


The Human TFome Library

The Human TFome Collection is the first comprehensive human transcription factor (TF) library and consists of over 1,700 human TF ORFs in Gateway shuttling vectors. Born out of a collaboration between the labs of George Church, Marc Vidal, and Jussi Taipale, the DNASU plasmid repository, and TransOMIC Technologies, the human TFome collection has been used to systematically identify TFs that induce differentiation of human stem cells. A portion of this collection is available through Addgene.

Combined image of two micrographs. On left, immunofluorescent stained functional neurons appear as a yellow web dotted with blue nuclei. On right, a tissue section of vascular endothelial cells appears as swirls of pink and purple on a gray background.
Figure 1. Functional neurons (left) and vascular endothelial cells (right) were derived from human pluripotent stem cells using the Human TFome technology. Image: Church Lab, Harvard Medical School
Combined image of two transmission electron micrographs of myelin in a cerebral organoid. On left, a grayscale image has a freeform oval delineated by multiple parallel lines. A yellow box around one region indicates the area magnified in the micrograph on right, where the striated cells are visible.
Figure 2. Myelin sheaths are shown wrapping around axons. Myelinating oligodendrocytes were produced by orthogonal programming of cerebral organoids using the Human TFome. Image: Church Lab, Harvard Medical School

Resources

Plasmids from Article

ID Plasmid Purpose
87977pDONR223-ZNF616Donor Vector containing ZNF616 transcription factor, part of the Human TFome Collection
87978pDONR223-ZNF3_isoform1Donor Vector containing ZNF3 transcription factor, part of the Human TFome Collection
87993pDONR223-ZNF540Donor Vector containing ZNF540 transcription factor, part of the Human TFome Collection
88002pDONR223-ZNF626Donor Vector containing ZNF626 transcription factor, part of the Human TFome Collection
88012pDONR223-ZNF268Donor Vector containing ZNF268 transcription factor, part of the Human TFome Collection
88019pDONR223-ZNF366Donor Vector containing ZNF366 transcription factor, part of the Human TFome Collection
88023pDONR223-ZNF473Donor Vector containing ZNF473 transcription factor, part of the Human TFome Collection
88024pDONR223-ZNF112 Donor Vector containing ZNF112 transcription factor, part of the Human TFome Collection
88029pDONR223-ZNF544Donor Vector containing ZNF544 transcription factor, part of the Human TFome Collection
88033pDONR223-ZNF41Donor Vector containing ZNF41 transcription factor, part of the Human TFome Collection
88037pDONR223-ZNF706Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection
88047pDONR223-ZNF484Donor Vector containing ZNF484 transcription factor, part of the Human TFome Collection
88065pDONR223-ZNF624Donor Vector containing ZNF624 transcription factor, part of the Human TFome Collection
88068pDONR223-ZNF786Donor Vector containing ZNF786 transcription factor, part of the Human TFome Collection
88070pDONR223-ZNF451Donor Vector containing ZNF451 transcription factor, part of the Human TFome Collection
88074pDONR223-ZNF576Donor Vector containing ZNF576 transcription factor, part of the Human TFome Collection
88081pDONR223-ZNF511Donor Vector containing ZNF511 transcription factor, part of the Human TFome Collection
88083pDONR223-ZNF423Donor Vector containing ZNF423 transcription factor, part of the Human TFome Collection
88091pDONR223-ZNF174Donor Vector containing ZNF174 transcription factor, part of the Human TFome Collection
88097pDONR223-ZNF695Donor Vector containing ZNF695 transcription factor, part of the Human TFome Collection
88102pDONR223-ZNF22Donor Vector containing ZNF22 transcription factor, part of the Human TFome Collection
88118pDONR223-ZNF219Donor Vector containing ZNF219 transcription factor, part of the Human TFome Collection
88127pDONR223-ZNF99Donor Vector containing ZNF99 transcription factor, part of the Human TFome Collection
88134pDONR223-ZNF747Donor Vector containing ZNF747 transcription factor, part of the Human TFome Collection
88143pDONR223-ZNF69Donor Vector containing ZNF69 transcription factor, part of the Human TFome Collection
88144pDONR223-ZNF607Donor Vector containing ZNF607 transcription factor, part of the Human TFome Collection
88157pDONR223-ZNF581Donor Vector containing ZNF581 transcription factor, part of the Human TFome Collection
88174pDONR223-ZNF277Donor Vector containing ZNF277 transcription factor, part of the Human TFome Collection
88188pDONR223-ZNF488Donor Vector containing ZNF488 transcription factor, part of the Human TFome Collection
88193pDONR223-ZNF346Donor Vector containing ZNF346 transcription factor, part of the Human TFome Collection
88245pDONR223-ZNF146Donor Vector containing ZNF146 transcription factor, part of the Human TFome Collection
88250pDONR223-ZNF397Donor Vector containing ZNF397 transcription factor, part of the Human TFome Collection
88279pDONR223-ZNF302Donor Vector containing ZNF302 transcription factor, part of the Human TFome Collection
88280pDONR223-ZNF3_isoform2Donor Vector containing ZNF3 transcription factor, part of the Human TFome Collection
88290pDONR223-ZNF662Donor Vector containing ZNF662 transcription factor, part of the Human TFome Collection
88304pDONR223-ZNF3_isoform3Donor Vector containing ZNF3 transcription factor, part of the Human TFome Collection
88305pDONR223-ZNF232Donor Vector containing ZNF232 transcription factor, part of the Human TFome Collection
88306pDONR223-ZNF410Donor Vector containing ZNF410 transcription factor, part of the Human TFome Collection
88309pDONR223-ZNF32Donor Vector containing ZNF32 transcription factor, part of the Human TFome Collection
88318pDONR223-ZNF645Donor Vector containing ZNF645 transcription factor, part of the Human TFome Collection
88320pDONR223-ZNF707Donor Vector containing ZNF707 transcription factor, part of the Human TFome Collection
88326pDONR223-ZNF558Donor Vector containing ZNF558 transcription factor, part of the Human TFome Collection
88327pDONR223-ZNF322Donor Vector containing ZNF322 transcription factor, part of the Human TFome Collection
88330pDONR223-ZNF621Donor Vector containing ZNF621 transcription factor, part of the Human TFome Collection
88331pDONR223-ZNF843Donor Vector containing ZNF843 transcription factor, part of the Human TFome Collection
88337pDONR223-ZNF670Donor Vector containing ZNF670 transcription factor, part of the Human TFome Collection
88341pDONR223-ZNF134Donor Vector containing ZNF134 transcription factor, part of the Human TFome Collection
88342pDONR223-ZNF830Donor Vector containing ZNF830 transcription factor, part of the Human TFome Collection
88349pDONR223-ZNF200Donor Vector containing ZNF200 transcription factor, part of the Human TFome Collection
88350pDONR223-ZNF764Donor Vector containing ZNF764 transcription factor, part of the Human TFome Collection
88355pDONR223-ZNF846Donor Vector containing ZNF846 transcription factor, part of the Human TFome Collection
88367pDONR223-ZNF562Donor Vector containing ZNF562 transcription factor, part of the Human TFome Collection
88368pDONR223-ZNF829_isoform1Donor Vector containing ZNF829 transcription factor, part of the Human TFome Collection
88370pDONR223-ZNF556Donor Vector containing ZNF556 transcription factor, part of the Human TFome Collection
88374pDONR223-ZNF79Donor Vector containing ZNF79 transcription factor, part of the Human TFome Collection
88377pDONR223-ZNF751 Donor Vector containing ZNF751 transcription factor, part of the Human TFome Collection
88396pDONR223-ZNF256Donor Vector containing ZNF256 transcription factor, part of the Human TFome Collection
88409pDONR223-ZNF71Donor Vector containing ZNF71 transcription factor, part of the Human TFome Collection
88415pDONR223-ZNF597Donor Vector containing ZNF597 transcription factor, part of the Human TFome Collection
88423pDONR223-ZNF689Donor Vector containing ZNF689 transcription factor, part of the Human TFome Collection
88433pDONR223-ZNF223Donor Vector containing ZNF223 transcription factor, part of the Human TFome Collection
88442pDONR223-ZNF331Donor Vector containing ZNF331 transcription factor, part of the Human TFome Collection
88444pDONR223-ZNF165Donor Vector containing ZNF165 transcription factor, part of the Human TFome Collection
88446pDONR223-ZNF658Donor Vector containing ZNF658 transcription factor, part of the Human TFome Collection
88451pDONR223-ZNF517Donor Vector containing ZNF517 transcription factor, part of the Human TFome Collection
88453pDONR223-ZNF449Donor Vector containing ZNF449 transcription factor, part of the Human TFome Collection
88455pDONR223-ZNF680Donor Vector containing ZNF680 transcription factor, part of the Human TFome Collection
88456pDONR223-ZNF502Donor Vector containing ZNF502 transcription factor, part of the Human TFome Collection
88459pDONR223-ZNF785 Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection
88461pDONR223-ZNF701Donor Vector containing ZNF701 transcription factor, part of the Human TFome Collection
88464pDONR223-ZNF582Donor Vector containing ZNF582 transcription factor, part of the Human TFome Collection
88465pDONR223-ZNF454Donor Vector containing ZNF454 transcription factor, part of the Human TFome Collection
88467pDONR223-ZNF257Donor Vector containing ZNF257 transcription factor, part of the Human TFome Collection
88470pDONR223-ZNF543Donor Vector containing ZNF543 transcription factor, part of the Human TFome Collection
88474pDONR223-ZNF345Donor Vector containing ZNF345 transcription factor, part of the Human TFome Collection
88479pDONR223-ZNF545 Donor Vector containing ZNF545 transcription factor, part of the Human TFome Collection
88480pDONR223-ZNF792_isoform2Donor Vector containing ZNF792 transcription factor, part of the Human TFome Collection
88481pDONR223-ZNF248Donor Vector containing ZNF248 transcription factor, part of the Human TFome Collection
88486pDONR223-ZNF752 Donor Vector containing ZNF752 transcription factor, part of the Human TFome Collection
88492pDONR223-ZNF404Donor Vector containing ZNF404 transcription factor, part of the Human TFome Collection
88493pDONR223-ZNF791Donor Vector containing ZNF791 transcription factor, part of the Human TFome Collection
88502pDONR223-ZNF155Donor Vector containing ZNF155 transcription factor, part of the Human TFome Collection
88504pDONR223-ZNF266Donor Vector containing ZNF266 transcription factor, part of the Human TFome Collection
88508pDONR223-ZNF19Donor Vector containing ZNF19 transcription factor, part of the Human TFome Collection
88513pDONR223-ZNF136Donor Vector containing ZNF136 transcription factor, part of the Human TFome Collection
88524pDONR223-ZNF768Donor Vector containing ZNF768 transcription factor, part of the Human TFome Collection
88526pDONR223-ZNF350Donor Vector containing ZNF350 transcription factor, part of the Human TFome Collection
88528pDONR223-ZNF8Donor Vector containing ZNF8 transcription factor, part of the Human TFome Collection
88529pDONR223-ZNF619Donor Vector containing ZNF619 transcription factor, part of the Human TFome Collection
88532pDONR223-ZNF774Donor Vector containing ZNF774 transcription factor, part of the Human TFome Collection
88539pDONR223-ZNF37ADonor Vector containing ZNF37A transcription factor, part of the Human TFome Collection
88543pDONR223-ZNF426Donor Vector containing ZNF426 transcription factor, part of the Human TFome Collection
88551pDONR223-ZNF175Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection
88554pDONR223-ZNF296Donor Vector containing ZNF296 transcription factor, part of the Human TFome Collection
88556pDONR223-ZNF10 Donor Vector containing ZNF10 transcription factor, part of the Human TFome Collection
88561pDONR223-ZNF23Donor Vector containing ZNF23 transcription factor, part of the Human TFome Collection
88562pDONR223-ZNF770Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection
88563pDONR223-ZNF132Donor Vector containing ZNF132 transcription factor, part of the Human TFome Collection
88567pDONR223-ZNF98Donor Vector containing ZNF98 transcription factor, part of the Human TFome Collection
88568pDONR223-ZNF416Donor Vector containing ZNF416 transcription factor, part of the Human TFome Collection
88573pDONR223-ZNF800Donor Vector containing ZNF800 transcription factor, part of the Human TFome Collection
88574pDONR223-ZNF778Donor Vector containing ZNF778 transcription factor, part of the Human TFome Collection
88578pDONR223-ZNF655Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection
88588pDONR223-ZNF720Donor Vector containing ZNF720 transcription factor, part of the Human TFome Collection
88589pDONR223-ZNF436Donor Vector containing ZNF436 transcription factor, part of the Human TFome Collection
88593pDONR223-ZNF195Donor Vector containing ZNF195 transcription factor, part of the Human TFome Collection
88594pDONR223-ZNF221Donor Vector containing ZNF221 transcription factor, part of the Human TFome Collection
88612pDONR223-ZNF753 Donor Vector containing ZNF753 transcription factor, part of the Human TFome Collection
88614pDONR223-ZNF554Donor Vector containing ZNF554 transcription factor, part of the Human TFome Collection
88615pDONR223-ZNF677Donor Vector containing ZNF677 transcription factor, part of the Human TFome Collection
88642pDONR223-ZNF35Donor Vector containing ZNF35 transcription factor, part of the Human TFome Collection
88643pDONR223-ZNF467Donor Vector containing ZNF467 transcription factor, part of the Human TFome Collection
88644pDONR223-ZNF574Donor Vector containing ZNF574 transcription factor, part of the Human TFome Collection
88649pDONR223-ZNF784Donor Vector containing ZNF784 transcription factor, part of the Human TFome Collection
88654pDONR223-ZNF837Donor Vector containing ZNF837 transcription factor, part of the Human TFome Collection
88658pDONR223-ZNF280CDonor Vector containing ZNF280C transcription factor, part of the Human TFome Collection
88664pDONR223-ZNF140Donor Vector containing ZNF140 transcription factor, part of the Human TFome Collection
88666pDONR223-ZNF698 Donor Vector containing ZNF698 transcription factor, part of the Human TFome Collection
88673pDONR223-ZNF660Donor Vector containing ZNF660 transcription factor, part of the Human TFome Collection
88678pDONR223-ZNF76Donor Vector containing ZNF76 transcription factor, part of the Human TFome Collection
88681pDONR223-ZNF30Donor Vector containing ZNF30 transcription factor, part of the Human TFome Collection
88684pDONR223-ZNF705DDonor Vector containing ZNF705D transcription factor, part of the Human TFome Collection
88688pDONR223-ZNF669Donor Vector containing ZNF669 transcription factor, part of the Human TFome Collection
88701pDONR223-ZNF692Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection
88703pDONR223-ZNF420Donor Vector containing ZNF420 transcription factor, part of the Human TFome Collection
88704pDONR223-ZNF792_isoform1Donor Vector containing ZNF792 transcription factor, part of the Human TFome Collection
88707pDONR223-ZNF829_isoform2Donor Vector containing ZNF829 transcription factor, part of the Human TFome Collection
88708pDONR223-ZNF781Donor Vector containing ZNF781 transcription factor, part of the Human TFome Collection
88710pDONR223-ZNF667Donor Vector containing ZNF667 transcription factor, part of the Human TFome Collection
88712pDONR223-ZNF776Donor Vector containing ZNF776 transcription factor, part of the Human TFome Collection
88721pDONR223-ZNF704Donor Vector containing ZNF704 transcription factor, part of the Human TFome Collection
88732pDONR223-ZNF230Donor Vector containing ZNF230 transcription factor, part of the Human TFome Collection
88740pDONR223-ZNF532Donor Vector containing ZNF532 transcription factor, part of the Human TFome Collection
88744pDONR223-ZNF77Donor Vector containing ZNF77 transcription factor, part of the Human TFome Collection
88753pDONR223-ZNF672Donor Vector containing ZNF672 transcription factor, part of the Human TFome Collection
88756pDONR223-ZNF804BDonor Vector containing ZNF804B transcription factor, part of the Human TFome Collection
88760pDONR223-ZNF285Donor Vector containing ZNF285 transcription factor, part of the Human TFome Collection
88761pDONR223-ZNF678Donor Vector containing ZNF678 transcription factor, part of the Human TFome Collection
88778pDONR223-ZNF589Donor Vector containing ZNF589 transcription factor, part of the Human TFome Collection
88779pDONR223-ZNF688_isoform1Donor Vector containing ZNF688 transcription factor, part of the Human TFome Collection
88783pDONR223-ZNF688_isoform2Donor Vector containing ZNF688 transcription factor, part of the Human TFome Collection
88785pDONR223-ZNF674Donor Vector containing ZNF674 transcription factor, part of the Human TFome Collection
88787pDONR223-ZNF688_isoform3Donor Vector containing ZNF688 transcription factor, part of the Human TFome Collection
88796pDONR223-ZNF324BDonor Vector containing ZNF324B transcription factor, part of the Human TFome Collection
88804pDONR221-ETV3Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection
88817pDONR221-ZNF7Donor Vector containing ZNF7 transcription factor, part of the Human TFome Collection
101398pDONR221-HOXB9Donor Vector containing HOXB9 transcription factor, part of the Human TFome Collection
101399pDONR221-HOXC5Donor Vector containing HOXC5 transcription factor, part of the Human TFome Collection
101402pDONR221-HOXD8Donor Vector containing HOXD8 transcription factor, part of the Human TFome Collection
101409pDONR221-HES5Donor Vector containing HES5 transcription factor, part of the Human TFome Collection
101410pDONR221-BSXDonor Vector containing BSX transcription factor, part of the Human TFome Collection
101411pDONR221-HES3Donor Vector containing HES3 transcription factor, part of the Human TFome Collection
101414pDONR221-ZNF705ADonor Vector containing ZNF705A transcription factor, part of the Human TFome Collection
101417pDONR221-GATA4Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection
101418pDONR221-GATA6Donor Vector containing GATA6 transcription factor, part of the Human TFome Collection
101429pDONR221-FOXE3Donor Vector containing FOXE3 transcription factor, part of the Human TFome Collection
101431pDONR221-FOXH1Donor Vector containing FOXH1 transcription factor, part of the Human TFome Collection
101439pDONR221-FOXE1Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection
101441pDONR221-ZNF860Donor Vector containing ZNF860 transcription factor, part of the Human TFome Collection
101445pDONR221-FOXN1Donor Vector containing FOXN1 transcription factor, part of the Human TFome Collection
101446pDONR221-ZNF732Donor Vector containing ZNF732 transcription factor, part of the Human TFome Collection
101447pDONR221-ZNF527Donor Vector containing ZNF527 transcription factor, part of the Human TFome Collection
101448pDONR221-ZNF665Donor Vector containing ZNF665 transcription factor, part of the Human TFome Collection
101449pDONR221-ZNF534Donor Vector containing ZNF534 transcription factor, part of the Human TFome Collection
101451pDONR221-ZNF251Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection
101454pDONR221-ZNF347Donor Vector containing ZNF347 transcription factor, part of the Human TFome Collection
101455pDONR221-ZNF442Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection
101460pDONR221-ZNF749Donor Vector containing ZNF749 transcription factor, part of the Human TFome Collection
101465pDONR221-ZNF730Donor Vector containing ZNF730 transcription factor, part of the Human TFome Collection
101466pDONR221-ZNF727Donor Vector containing ZNF727 transcription factor, part of the Human TFome Collection
101470pDONR221-ZNF808Donor Vector containing ZNF808 transcription factor, part of the Human TFome Collection
101472pDONR221-ZNF835Donor Vector containing ZNF835 transcription factor, part of the Human TFome Collection
101475pDONR221-ZNF682Donor Vector containing ZNF682 transcription factor, part of the Human TFome Collection
101478pDONR221-HOXB3Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection
101481pDONR221-ZNF716Donor Vector containing ZNF716 transcription factor, part of the Human TFome Collection
101487pDONR221-ZNF275Donor Vector containing ZNF275 transcription factor, part of the Human TFome Collection
101491pDONR221-FOXD4L3Donor Vector containing FOXD4L3 transcription factor, part of the Human TFome Collection
101492pDONR221-FOXD4L5Donor Vector containing FOXD4L5 transcription factor, part of the Human TFome Collection
101502pDONR221-ZNF66Donor Vector containing ZNF66 transcription factor, part of the Human TFome Collection
101504pDONR221-ZNF705BDonor Vector containing ZNF705B transcription factor, part of the Human TFome Collection
101505pDONR221-ZNF705GDonor Vector containing ZNF705G transcription factor, part of the Human TFome Collection
101508pDONR221-ZNF441Donor Vector containing ZNF441 transcription factor, part of the Human TFome Collection
101510pDONR221-FOXD4L1Donor Vector containing FOXD4L1 transcription factor, part of the Human TFome Collection
101514pDONR221-ZNF799Donor Vector containing ZNF799 transcription factor, part of the Human TFome Collection
101515pDONR221-ASCL3Donor Vector containing ASCL3 transcription factor, part of the Human TFome Collection
101518pDONR221-FOXI3Donor Vector containing FOXI3 transcription factor, part of the Human TFome Collection
101533pDONR221-ZNF852_isoform1Donor Vector containing ZNF852 transcription factor, part of the Human TFome Collection
101547pDONR221-ZNF197Donor Vector containing ZNF197 transcription factor, part of the Human TFome Collection
101549pDONR221-ZNF208Donor Vector containing ZNF208 transcription factor, part of the Human TFome Collection
101558pDONR221-ZNF233Donor Vector containing ZNF233 transcription factor, part of the Human TFome Collection
101560pDONR221-ZNF681Donor Vector containing ZNF681 transcription factor, part of the Human TFome Collection
101564pDONR221-ZNF470Donor Vector containing ZNF470 transcription factor, part of the Human TFome Collection
101565pDONR221-ZNF507Donor Vector containing ZNF507 transcription factor, part of the Human TFome Collection
101566pDONR221-ZNF516Donor Vector containing ZNF516 transcription factor, part of the Human TFome Collection
101567pDONR221-ZNF518BDonor Vector containing ZNF518B transcription factor, part of the Human TFome Collection
101569pDONR221-ZNF579_isoform2Donor Vector containing ZNF579 transcription factor, part of the Human TFome Collection
101589pDONR221-ZNF805Donor Vector containing ZNF805 transcription factor, part of the Human TFome Collection
101595pDONR221-ZNF735Donor Vector containing ZNF735 transcription factor, part of the Human TFome Collection
101602pDONR221-ZNF736Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection
101608pDONR221-ZNF883Donor Vector containing ZNF883 transcription factor, part of the Human TFome Collection
101611pDONR221-ZNF234Donor Vector containing ZNF234 transcription factor, part of the Human TFome Collection
101615pDONR221-ZNF385ADonor Vector containing ZNF385A transcription factor, part of the Human TFome Collection
101621pDONR221-ZNF587BDonor Vector containing ZNF587B transcription factor, part of the Human TFome Collection
101622pDONR221-ZNF222Donor Vector containing ZNF222 transcription factor, part of the Human TFome Collection
101623pDONR221-ZNF879Donor Vector containing ZNF879 transcription factor, part of the Human TFome Collection
101625pDONR221-ZNF646Donor Vector containing ZNF646 transcription factor, part of the Human TFome Collection
101628pDONR221-FOXQ1Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

Antibodies from Article