Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast.
Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H
ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87941 | pHES839 | Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites. |
| 87942 | pHES941 | Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites. |
| 87943 | pHES795 | Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites. |
| 87944 | pHES830 | Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites. |
| 87945 | pHES873 | Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites. |
| 87946 | pHES835 | Expresses YFP under control of synthetic promoter containing Zif268 binding sites. |
| 87947 | pHES834 | Expresses mKate2 under control of GAL1 promoter. |
| 89190 | pHES820 | Expresses YFP under control of GAL1 promoter. |
| 89191 | pHES840 | Expresses YFP under control of GAL1 promoter. |
| 89192 | pHES875 | Expresses mCherry under control of GAL1 promoter. |
| 89193 | pHES822 | Expresses YFP under control of synthetic promoter containing Zif268 binding sites. |
| 89194 | pHES841 | Expresses YFP under control of synthetic promoter containing Zif268 binding sites. |
| 89195 | pHES836 | Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites. |
| 89196 | pHES874 | Expresses mCherry under control of synthetic promoter containing Zif268 binding sites. |
| 89197 | pHES854 | Expresses mKate2 under control of TDH3 constitutive promoter. |
| 89199 | pHES936 | Expresses DIG1 under control of GAL1 promoter |
| 89200 | pHES938 | Expresses MSG5 under control of GAL1 promoter |
| 89201 | pHES939 | Expresses GPA1 under control of GAL1 promoter |
| 89202 | pHES940 | Expresses STE4 under control of synthetic promoter containing Zif268 binding sites. |
| 90408 | pHES942 | Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites. |
| 90409 | pHES833 | Expresses YFP under control of GAL1 promoter |